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FISV 2006 .::. 8th Annual Meeting
Riva del Garda, 28 September - 1 October 2006

Abstract for session
Minisymposia II
30/09/2006, 08:30 - [---]
Plant mineral nutrition and metabolism
(Organisers: A. Marocco, G.A. Sacchi)



Transcript co-regulation for identifying genes involved in glucosinolates metabolism and regulation in Arabidopsis

M. Bertossi1, L. Mizzi2, P.A. Morandini1
1Dept Biology, Univ. Milan (Milan);
2Dept Biomolecular Sciences and Biotechnology, Univ. Milan (Milan)

Glucosinolates (GI) are a small group of group of secondary metabolites derived from aminoacids and containing N and S. We use transcript co-regulation (from public microarray data) to identify genes involved in the pathway or its regulation. Several genes are co-regulating with the enzymes involved in the three major GI pathways from Arabidopsis (Trp, Phe and Homomet). We can suggest candidates for: i. unidentified enzymatic steps, ii. transporters involved in GI movement, iii. pathway regulators. Candidate genes identified are: A) Several Glutathione S-transferases as donor for the reduced sulfur. B) Two aminotransferases acting on methionine as a first step either prior to or after side chain elongation. C) Three aconitases-like proteins (At3g58990, At4g13430 and At2g43100) involved in the hydroxyl migration during chain elongation in homomethione derived GI. D) PEN3 (At1g59870) as the ABC transporter of Trp-derived GI across the plasma membrane and PEN2 (At2g44490) as the glycosyl hydrolase family 1 protein involved in their transport and hydrolysis at infection sites. E) A transporter acting on methionine-derived GI. F) Several MYB genes as regulators.